Paper sheets made from sugarcane bagasse and lemongrass by-products: Synthesis and properties

Vietnam J. Agri. Sci. 2021, Vol. 19, No. 7: 964-974  
Tạp chí Khoa học Nông nghiệp Việt Nam 2021, 19(7): 964-974  
PAPER SHEETS MADE FROM SUGARCANE BAGASSE  
AND LEMONGRASS BY-PRODUCTS: SYNTHESIS AND PROPERTIES  
Ngo Thi Thuong1, Tran Thi Thuy Dung2, Chu Thi Thanh1,  
Nguyen Thi Hong Hanh1, Le Thi Thu Huong1*  
1Department of Chemistry, Faculty of Environment, Vietnam National University of Agriculture,  
2Student at K61CNTPA class, Faculty of Food Science and Technology,  
Vietnam National University of Agriculture  
*Correspondence to: [email protected]  
Received: 02.03.2020  
Accepted: 04.03.2021  
ABSTRACT  
Plastic single-use packaging is one of the largest contributors to plastic pollution in Vietnam as well as in many  
other countries. Alternative materials, especially materials derived from natural and renewable sources, should be  
developed to solve this global issue. In this study, we aimed to investigate the synthesis and properties of packaging  
paper sheets from sugarcane bagasse and lemongrass by-products. The delignification of the biomass was  
implemented at different NaOH/biomass ratios and hydrolysis times while the paper making process was studied at  
various sugarcane bagasse/lemongrass ratios and different amounts of glycerol and starch additives. The obtained  
paper sheets were then tested for their mechanic properties and water absorption through ASTM (American Society  
for Testing and Materials) procedures, and the biodegradability by scanning electron microscopy (SEM). The results  
showed that the paper sheets at the optimized conditions had low thickness (0.3mm), density (0.4 g cm-3), and water  
absorption but high tensile strength (19 N mm-2) and flexural modulus (17 N). These properties and their  
biodegradability suggest that the paper sheets could potentially be used in packaging.  
Keywords: Biodegradability, lemongrass, optimization, paper sheet making, sugarcane bagasse, tensile strength.  
There is  
a
lot of agricultural waste  
1. INTRODUCTION  
containing fiber in Vietnam such as sugarcane  
bagasse, lemongrass bagasse (by-product of  
lemongrass after essential oil distillation), rice  
straw, banana pseudostems and leaves, and  
Zingiberaceae leaves. Sugarcane bagasse  
compositions are mainly cellulose (about 60%),  
hemicellulose (20%), glucose (10%), and some  
other ingredients. Although sugarcane bagasse  
is considered the ideal raw material for  
producing many new products due to its  
abundant supply and stable price, it needs to be  
modified to obtain the desired mechanical  
properties. For example, cellulosic fibers from  
bagasse were combined with powders to form  
composite materials; combined with gelatin,  
starch, and agar for making tableware; or mixed  
with wood pulp resins and other natural fibers  
(Loh et al., 2013). The cellulose content in  
White pollution or plastic pollution has  
become  
a global problem that has many  
severely negative impacts on humans and the  
ecosystem. One of the biggest sources of plastic  
pollution is about 400 million tons of single-use  
packaging that accounts for 36% of the total  
plastic production annually (UNEP, 2018). It is  
urgent to find out alternative sustainable  
materials to replace plastic in the packaging  
industry. Renewable agricultural by-product  
fibers have attracted much attention from  
researchers worldwide to serve as substitutes  
for petroleum-based polymers which can be  
highly contaminated by hazardous substances  
such as PAH (Rochman et al., 2013), PCB  
(Pascall et al., 2005) or heavy metals (Alam et  
al., 2018a; Alam et al., 2018b).  
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Ngo Thi Thuong, Tran Thi Thuy Dung, Chu Thi Thanh, Nguyen Thi Hong Hanh, Le Thi Thu Huong  
lemongrass by-products is about 40% (Kaur &  
2. MATERIALS AND METHODS  
Dutt, 2013). When combined with sugarcane  
bagasse, the difference in cellulose content and  
polymeric chain lengths of the different fibers  
may enhance some mechanical properties of the  
obtained composites (Agustina et al., 2019).  
2.1. Raw materials preparation  
The bagasse and lemongrass by-products  
were collected in Hanoi. The collected materials  
were dried in an oven (42°C) for 3 days, cut into  
small pieces with average lengths of ~3cm,  
again dried at 42°C overnight, and then kept in  
plastic seal pack bags until further utilization.  
It was also published that fibers from  
agricultural by-products could be used to  
make paper sheets. In Oman, Khalsa Al-  
Sulaimani and his research group (Al-  
Sulaimani et al., 2017) produced handmade  
paper from bagasse and banana stalk fiber.  
The research team investigated three  
composition formulas: formula 1 had only raw  
materials from bagasse and banana, formula 2  
and 3 were fibers mixed with CaCO3 fillers  
(2% and 5%), respectively, and starch (2%-5%)  
for adhesion. The research results showed that  
adding additives increased adhesion and the  
whiteness of the paper while reducing the  
paper thickness. Bagasse is more rigid than  
banana fiber so it is suitable for wrapping  
paper, while banana fiber is very suitable for  
making soft paper such as tissue paper.  
Delignification (Isolation of lignin from  
biomass)  
To produce paper sheets, the biomass was  
first delignified to separate the fibers from the  
lignin, a group of substances that attach the  
fibers with each other. To determine the  
optimized parameters of delignification, the  
samples were treated with NaOH at different  
ratios (from 4 to 20% NaOH/biomass) and  
different NaOH concentrations (1M to 5M) and  
the samples were denoted as TN1.1 to TN1.4.  
The samples were treated for various periods of  
time (from 0.5h to 2h) corresponding to samples  
TN2.1 to TN2.4. After the delignification, the  
solid fractions were separated by muslin cloth,  
rinsed with water until neutral, and dried in  
the oven (42°C) for 3 days. The solid fractions  
were weighed before being mixed with 1L of  
water and grinding to form a pulp mixture. The  
paper making yield (%) was determined by  
Formula (1):  
Although these fibers are important  
resources, there are few environmental-friendly  
applications of these materials in use now in  
Vietnam. The major quantity of sugarcane  
bagasse in Vietnam is used for low value  
thermal purposes resulting in carbon release.  
Sugarcane and lemon bagasse have been  
sometimes used as microbiological fertilizer  
(Pham, 2017) or animal feed (Hoang, 2017).  
However, huge amounts of these by-products  
are discharged into the environment or burned,  
resulting in a waste of resources and increased  
greenhouse gas emissions.  
Making yield (%) = Paper sheet weight/  
Delignified biomass weight 100 (1)  
2.2. Paper Making  
The above-mentioned pulp was used to  
make sheets of paper. In this step, additives  
(glycerol and starch) were added to the pulp and  
the ratios of the biomass were varied to enhance  
the properties of the paper. Glycerol was added  
at the amounts of 0, 2, 4, 6, and 8ml for 50g of  
initial biomass (TN3.1 to TN3.5). The mass  
ratios of the sugarcane bagasse and lemongrass  
by-products were 100-0, 90-10, 80-20, and  
70-30 (TN4.1 to TN4.4). Starch was added at 2,  
4, and 6% for 2 ratios of the biomass (TN5.1 to  
TN5.6)  
In our previous initial study, we found  
that sugarcane and lemongrass bagasse could  
be combined to generate bio composites (Ngo et  
al., 2018). In this study, we aimed to  
systematically investigate the synthesis and  
properties of paper sheets made by different  
combinations of sugarcane bagasse and  
lemongrass by-products.  
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Paper sheets made from sugarcane bagasse and lemongrass by-products: Synthesis and properties  
.
Figure 1. Synthesis and characterization diagram of the paper sheets  
2.3. Paper testing  
2.3.3. Tensile strength and flexural  
modulus  
Based on ASTM D638 and ASTM D790, the  
tensile strength and flexural modulus were  
measured in a universal testing machine. Three  
paper bands of 210 cm for each sample were  
tested to determine the average values.  
The pH, thickness, density, tensile  
strength, flexural modulus, and water  
absorption were determined from the obtained  
paper sheets according to respective ASTM. In  
addition, the biodegradability test was carried  
out according to the published procedure of  
Marichelvam et al. (2019).  
2.3.4. Water absorption  
2.3.1. pH test  
Water absorption of the paper sheets was  
determined at room conditions (ASTM D570-  
98). The paper pieces of 5 2cm were weighed  
before (wi) and after (wf) the water absorption  
process. Each sample was tested 3 times to get  
an average value. The percent of absorbed  
water was calculated by Formula (3):  
To determine the pH of the paper sheets,  
3 3cm pieces were immersed in 50ml of  
distilled water for 5h. The pH values of the  
solutions were then measured with a pH meter  
(HANNA HI98107).  
2.3.2. Thickness and density determination  
% wwater = [(wf – wi)/wi] 100  
(3)  
The thicknesses of paper pieces sized 2   
2cm were determined with an electronic caliper  
(Mitutoyo) (Figure 1). The pieces were also  
weighed in an analytical balance. The density of  
each sample was calculated by Formula (2):  
where wwater is the weight percentage of the  
water absorbed by the paper, wi is the weight of  
the dry paper sample, and wf is the weight of  
the saturated paper sample.  
2.3.5. Biodegradability Test  
m
22h  
d   
(2)  
The biodegradability of the best sample  
(paper with high tensile strength, low density,  
and low water absorption) was determined. This  
sample was cut into pieces of 2 2cm. Soil  
(500g) from a soybean field with a slight  
in which d, m, and h are the density  
(g cm-3), the mass (g), and the thickness (cm) of  
the sample, respectively.  
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Ngo Thi Thuong, Tran Thi Thuy Dung, Chu Thi Thanh, Nguyen Thi Hong Hanh, Le Thi Thu Huong  
moisture content was collected and stored in a  
fibers. In sugarcane bagasse and lemongrass  
by-products, in addition to the main component  
of cellulose, there are also other components of  
hemicellulose and lignin. Therefore, it was  
necessary to use alkali to separate the  
hemicellulose and lignin from the biomass.  
Sodium hydroxide solution can separate lignin,  
hemicellulose, and other solutes (sugar, ash,  
wax, and protein, among others) from biomass.  
NaOH is capable of breaking down cell walls  
because the alkali dissolves the lignin and  
hemicellulose and also breaks down the α-ether  
links between the lignin and hemicellulose. The  
remaining lignin in the pulp makes it become  
dark yellow or brown (affecting the sensory  
value of the paper) and hard (Kido, 2016).  
Therefore, the ratio of NaOH/biomass is an  
important factor affecting the hydrolysis of the  
biomass. For a certain amount of biomass, if too  
little NaOH is used, it will not be enough to  
thoroughly remove the lignin. Conversely, if the  
amount of NaOH used is too high, when the  
hydrolysis process has ended, the remaining  
NaOH will lead to waste of chemicals and water  
to neutralize the biomass. Therefore, the  
NaOH/biomass ratio was first investigated. The  
results are shown in Table 1.  
container. Three pieces of the paper sample  
were buried inside the soil at a depth of 5cm  
and 3 others were buried at a depth of 10cm for  
15 days at room conditions. The weights of the  
pieces were measured before and after the  
testing. The biodegradability of each sample  
was calculated by Formula (4):  
Weight Loss (%) = [(wo − w)/wo] × 100, (4)  
where wo and w are the average masses of  
the pieces before and after the test, respectively.  
The microstructures of the samples before  
and after the biodegradability test were  
recorded by scanning electron microscopy (SEM)  
on an FEI Nova NanoSEM system.  
2.4. Data analysis  
Data were processed in Excel 2010,  
Mathlab 2016, and Origin 8.0 software. One-  
way Anova analysis was performed to compare  
samples with significant differences at p <0.05.  
3. RESULTS AND DISCUSSION  
3.1. Delignification  
The main component of paper is cellulose  
3.1  
3.2  
3.3  
3.4  
3.5  
3.1  
3.2  
3.3  
3.4  
3.5  
(a)  
(b)  
3.1  
3.2  
3.3  
3.4  
3.5  
(c)  
Figure 2. Water absorption of the TN3 series at (a) 15s, (b) 30s, and (c) 1 min  
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Paper sheets made from sugarcane bagasse and lemongrass by-products: Synthesis and properties  
Table 1. Effects of the NaOH/biomass ratio on delignification  
NaOH/  
biomass  
ratio (%)  
Delignified  
biomass  
weight (g)  
Paper sheet  
weight (g)  
Papermaking  
yield (%)  
Thickness  
(mm)  
Sample  
TN1.1  
Obtained fibers  
Obtained paper sheet  
4
42.75a ±  
0.26  
34.06a ± 0.36  
80.9 ± 1.0  
92.7 ± 0.8  
91.2 ± 1.1  
89.1 ± 1.1  
1.61a ± 0.38 Difficult to grind,  
hard fibers with  
the size range of  
2-2.5 cm  
TN1.2  
TN1.3  
TN1.4  
10  
14  
20  
32.90b ±  
0.15  
30.38b ± 0.23  
1.41a ± 0.24 Less difficult to  
grind, hard fibers  
with the size  
range of 1.5-2 cm  
28.80c ± 0.18 26.14c ± 0.27  
1.11b ± 0.08 Easy to grind,  
soft and short  
fibers  
28.49c ± 0.07 25.26c ± 0.32  
1.05b ± 0.16 Easy to grind,  
soft and short  
fibers  
Note: Values with the same letter within each column are not significantly different at the 5% level.  
The results of Table 1 show that, when the  
ratio of NaOH/biomass increased from 4 to 20%,  
the remaining biomass weight after hydrolysis  
decreased. When this weight increased to 20%,  
the remaining amount of biomass decreased  
insignificantly compared to that at the ratio of  
14%. This reveals that the 14% ratio is enough  
to hydrolyze the biomass.  
However, in the samples TN1.2 and TN1.1, the  
cellulose fiber bundles were difficult to split,  
and the raw fibers were clearly visible on the  
finished paper. Meanwhile, samples TN1.3 and  
TN1.4 had much better fineness. Thus,  
combining  
the  
above  
parameters,  
the  
NaOH/biomass ratio of 14% (TN1.3) was  
selected to carry out the next studies.  
In addition, the paper-making yield was  
only different when the NaOH/biomass ratio  
used was 4%. The reason for this is that the  
low paper yield was due to the fact that the  
cellulosic fibers had not been separated due to  
an incomplete lignin reaction, thus their  
ability to bond when paper-making was low. In  
the remaining three cases, the paper making  
yields were not significantly different, and  
about 90% of the weight of the delignified  
biomass could be converted into a paper sheet.  
The effects of hydrolysis time on the  
lignification (Table 2) show that when the  
hydrolysis time increased, the weight of the  
delignified biomass weight decreased, in which  
there was no observed significant difference  
between TN2.3 and TN2.4. The paper sheet  
yield did not vary much among the samples,  
however, the resulting paper had a good  
smoothness in the TN2.3 and TN2.4 formulas.  
Thus, we chose these two samples to perform  
hydrolysis for 2h in subsequent tests. In a  
968  
Ngo Thi Thuong, Tran Thi Thuy Dung, Chu Thi Thanh, Nguyen Thi Hong Hanh, Le Thi Thu Huong  
published study involving the sugarcane  
15s in contact with water, the paper bands  
absorbed water more than 50% of their weight.  
After 30 s, they absorbed 100% of the water. At  
the contact time of 15s, TN3.2 (2ml glycerol  
added) was the least water absorbent sample.  
However, after 60s, the water absorbed  
amounts in TN3.2, TN3.3, and TN3.4 became  
close to that of each other. These differences  
were not statistically significant. TN3.1 and  
TN3.5 showed the highest water absorption.  
This can be explained by the fact that without  
glycerol (TN3.1), water was more easily able to  
go into the cellulose fibers in the biomass, while  
with too much glycerol (TN3.5), water could be  
absorbed into the paper sheets through the  
glycerol dissolution process. These results  
suggested that 4 ml of glycerol was enough to  
absorb and connect the fibers in the biomass.  
However, based on the three properties of  
tensile strength, flexural modulus (Table 3),  
and absorption, the TN3.4 condition (6ml  
glycerol) was chosen to proceed with other  
experiments.  
bagasse/starch/PVA composite, the NaOH  
treatment changed the surface of the fibers  
which in turn changed the wettability of the  
samples (Zhu et al., 2019).  
3.2. Papermaking process  
3.2.1. Effects of the glycerol amount on the  
paper properties  
Adding glycerol (2, 4, 6, or 8ml) to the pulp  
made it easier to form paper sheets with a much  
smaller thickness compared to the sample  
without glycerol (Table 3). Table 3 also shows  
that the added amount of glycerol had effects on  
the tensile strength and flexural modulus.  
However, 2ml of glycerol (TN3.2) was not  
enough to significantly change the tensile  
strength and flexural modulus for the obtained  
paper compared to the sample without glycerol.  
When the added glycerol amount increased to 4  
ml and higher, the tensile strength and flexural  
modulus  
of  
these  
samples  
increased  
significantly compared to the TN3.1 and TN3.2  
samples. Among samples TN3.3, TN3.4, and  
TN3.5, there were some significant changes in  
the tensile strength and flexural modulus.  
Increasing the glycerol amount appeared to  
raise the tensile strength and flexural modulus  
of the samples. It has been reported that  
glycerol could lubricate the cellulose fibers to  
make the links between cellulose fibers tighter,  
resulting in pulp that was then easier to make  
into paper with a smaller thickness, and greater  
density, flexural strength, and tensile strength  
(Cruz et al., 2017). Although, glycerol is water-  
soluble, in order to select the appropriate  
glycerol ratio, it was necessary to further  
consider the water absorption properties of the  
paper. Water absorption is greatly affected by  
the structural integrity of the paper. The cause  
of this phenomenon is that the natural  
capillaries found in bagasse make the paper  
quickly reach hydration equilibrium. The  
results of the water absorption test of this  
sample series are presented in Figure 2 and  
Figure 3. When not using glycerol, after only  
3.2.2. Effects of the sugarcane bagasse/  
lemongrass ratio on the paper properties  
Table 4 shows the effect of the lemongrass  
composition on the paper properties. It was  
obvious that when adding lemongrass, the  
paper sheets became thinner with a higher  
density, higher tensile strength, and higher  
flexural modulus. The differences between the  
strength of the cellulose fibers (due to the  
length of the cellulose chains) of the sugarcane  
bagasse and lemongrass could be the reason for  
the significant changes in these parameters  
between TN4.2, TN4.3, or TN4.4 and TN4.1.  
Figure 4 also demonstrates that the higher the  
ratio of lemongrass, the less water was  
absorbed into the paper sheets. These results  
also suggested that the combination could be an  
effective approach to controlling the paper  
properties. In our previous study, the composite  
samples with lower contents of lemongrass also  
showed lower tensile strength and flexural  
modulus (Ngo et al., 2018). For further  
experiments, the sugarcane bagasse/lemongrass  
969  
Paper sheets made from sugarcane bagasse and lemongrass by-products: Synthesis and properties  
ratios of 80/20 and 70/30 were chosen. Another  
study showed a similar dependence of the  
composite mechanical properties on their  
composition (Agustina et al., 2019). Different  
ratio mixtures of sugarcane bagasse and  
pineapple leaves were also investigated to  
prepare paper pulp resulting in papers with  
varied tensile and tearing strengths (Evelyn et  
al., 2019).  
experiment series did not show much variation.  
The results in Table 5 reveal that adding starch  
at the ratios of 4% and 6% for both ratios of  
biomass (80/20 and 70/30) made the tensile  
strength and flexural modulus of the samples  
significantly (P < 0.5) greater than that at the  
ratio of 2%. This showed that when starch was  
used, the links between the cellulose fibers  
increased leading to stronger and more flexural  
paper. The tensile strength and flexural modulus  
of the samples at the ratios of 4% and 6% starch  
were not significantly different at the 5%  
significance level. There ws a similar trend in the  
water absorption of the samples (Figure 5).  
3.2.3. Effects of the added starch amount  
on the paper properties  
All the researched samples were neutral.  
The thickness and density of the samples in this  
Figure 3. Effect of the added glycerol amount on the water adsorption capacity  
Figure 4. Effects of the sugarcane bagasse/lemongrass  
on the water absorption of the paper sheets  
970  
Ngo Thi Thuong, Tran Thi Thuy Dung, Chu Thi Thanh, Nguyen Thi Hong Hanh, Le Thi Thu Huong  
Figure 5. Effects of the starch amount on the water absorption of the paper sheets  
Table 2. Effects of reaction time on the delignification of the biomass  
Delignified biomass Paper sheet Papermaking Thickness  
Sample Time (h)  
Obtained fibers  
Obtained paper sheet  
weight (g)  
weight (g)  
yield (%)  
(cm)  
TN2.1  
TN2.2  
0.5  
29.43 ± 0.32  
28.96 ± 0.12  
92.1 ± 1.1  
1.13a ± 0.20 Difficult to grind,  
hard fibers with  
the size range of  
2-2.5cm  
1
27.18 ± 0.21  
24.52 ± 0.17  
27.00 ± 0.25  
24.33 ± 0.13  
99.3 ± 1.2  
0.94a ± 0.26 Less difficult to  
grind, hard fibers  
with the size range  
of 1.5-2cm  
TN2.3  
TN2.4  
2
3
99.2 ± 0.9  
0.77b ± 0.11 Easy to grind, soft  
and short fibers  
24.03 ± 0.18  
23.71 ± 0.10  
98.7 ± 0.8  
0.73b ± 0.10 Easy to grind, soft  
and short fibers  
Note: Values with the same letter within each coulmn are not significantly different at the 5% level.  
Therefore, 4% starch was an appropriate  
amount for the papermaking process. The data  
showed that if the links between cellulose fibers  
were too tight, then the tensile and flexural  
strength would increase very little when more  
starch was used. The ratio of 4% was the ratio  
of the flexural strength and the tensile strength  
of the hearing.  
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Paper sheets made from sugarcane bagasse and lemongrass by-products: Synthesis and properties  
49.8% weight losses indicated the biodegradation  
3.3. Biodegradability properties  
of the samples. The SEM images (Figure 6) of the  
samples also showed a decrease in the thickness  
of the samples. There was a difference in the  
biodegradability rate between the 2 depths. This  
result differs from those published by  
Mirachelvam et al. (2019). This could be due to  
the differences in the composition of the samples  
in the current study and referent research.  
The biodegradability test results are shown  
in Figure 6. The optimized sample pieces of  
TN5.5 were buried in soil at different depths of  
5 cm and 10 cm. The initial average weight of  
the TN5.5 pieces was 0.0562 ± 0.0017. After 15  
days, the weights of the TN5.5 pieces at 5 cm  
and 10 cm-depths were 0.0367 ± 0.0015 and  
0.0282 ± 0.0018g, respectively. These 34.7% and  
Table 3. Effects of added glycerol amount on the paper properties  
Glycerol amount  
(ml)  
Tensile strength  
(N mm-2)  
Flexural  
modulus (N)  
Formula  
pH  
Thickness (mm)  
density (g cm-3)  
TN3.1  
TN3.2  
TN3.3  
TN3.4  
TN3.5  
0
2
4
6
8
6.4  
6.3  
6.5  
6.5  
6.4  
0.75a ± 0.05  
0.48b ± 0.05  
0.47b ± 0.04  
0.46b ± 0.04  
0.46b ± 0.08  
0.28 ± 0.03  
0.35 ± 0.06  
0.32 ± 0.06  
0.34 ± 0.04  
0.34 ± 0.09  
6.30a ± 0.10  
8.23b ± 0.05  
14.33c ± 0.11  
17.70d ± 0.70  
24.00e ± 0.81  
0.61a ± 0.10  
0.70a ± 0.10  
0.93b ± 0.05  
1.10bc ± 0.10  
1.23c ± 0.05  
Note: Values with the same letter within each column are not significantly different at the 5% level.  
Table 4. Effects of the sugarcane bagasse and lemongrass byproduct ratio  
on the paper properties  
Sugarcane  
bagasse/lemongrass  
by-product ratio  
Thickness  
(mm)  
density  
(g cm-3)  
Tensile strength  
(N mm-2)  
Flexural modulus  
(N)  
Formula  
pH  
TN4.1  
TN4.2  
TN4.3  
TN4.4  
100/0  
90/10  
80/20  
70/30  
6.3  
6.3  
6.2  
6.3  
0.46 ± 0.09a  
0.29 ± 0.03b  
0.30 ± 0.03b  
0.33 ± 0.03b  
0.31 ± 0.09  
0.48 ± 0.07  
0.42 ± 0.05  
0.43 ± 0.07  
16.27a ± 2.04  
18.07ab ± 1.72  
20.43bc ± 0.81  
21.46c ± 0.33  
1.13a ± 0.06  
1.31b ± 0.10  
1.33b ± 0.06  
1.43b ± 0.05  
Note: Values with the same letter within each column are not significantly different at the 5% level.  
Table 5. Effects of the added starch amount on the paper properties  
Sugarcane  
bagasse/lemongrass  
by-product ratio  
Starch  
amount  
(%)  
Tensile  
strength  
(N mm-2)  
Thickness  
(mm)  
density  
(g cm-3)  
Flexural modulus  
(N)  
Formula  
pH  
TN5.1  
TN5.2  
TN5.3  
TN5.4  
TN5.5  
TN5.6  
80/20  
80/20  
80/20  
70/30  
70/30  
70/30  
2
4
6
2
4
6
6.3  
6.4  
6.4  
6.3  
6.4  
6.3  
0.32a ± 0.01 0.43 ± 0.05  
0.30a ± 0.01 0.50 ± 0.04  
0.30a ± 0.02 0.44 ± 0.07  
0.31 a± 0.01 0.48 ± 0.03  
0.32a ± 0.01 0.44 ± 0.04  
0.33a ± 0.02 0.39 ± 0.02  
11.87a ± 0.31  
18.33b ± 0.15  
18.80b ± 0.41  
16.30c ± 0.10  
18.90b ± 0.36  
19.60b ± 0.56  
0.93a ± 0.15  
1.17ab ± 0.15  
1.31b ± 0.10  
1.32b ± 0.11  
1.63c ± 0.11  
1.77c ± 0.06  
Note: Values with the same letter within each column are not significantly different at the 5% level.  
972  
Ngo Thi Thuong, Tran Thi Thuy Dung, Chu Thi Thanh, Nguyen Thi Hong Hanh, Le Thi Thu Huong  
Figure 6. SEM images of the TN5.5 pieces before (a)  
and after the biodegradability test at 5-cm (b) and 10-cm depth (c)  
possible to prepare bio bags or bio containers  
from the optimized paper sheets.  
4. CONCLUSIONS  
In conclusion, the optimized conditions of  
the synthesis of paper sheets from sugarcane  
bagasse and lemongrass by-products have been  
investigated. To obtain paper sheets with high  
tensile strength and flexural modulus, and with  
low thickness and water absorption, the  
biomass should be treated with 14% NaOH (w/w  
ACKNOWLEDGEMENTS  
This work was financially supported by  
Vietnam National University of Agriculture  
under the Grant No.T2019-04-19.  
to  
biomass)  
for  
2h.  
The  
sugarcane  
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mm-2, and flexural modulus of 1.7 N. The  
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49.8% in the soil layer at a 10 cm depth after 15  
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